Fate of mouse macrophages radiolabelled with PKH-95 and injected intravenously.
Identifieur interne : 004716 ( Main/Exploration ); précédent : 004715; suivant : 004717Fate of mouse macrophages radiolabelled with PKH-95 and injected intravenously.
Auteurs : RBID : pubmed:8535344English descriptors
- KwdEn :
- Animals, Cell Survival, Cells, Cultured, Erythrocytes (cytology), Erythrocytes (physiology), Fluorescent Dyes (diagnostic use), Fluorescent Dyes (pharmacokinetics), Indium Radioisotopes (diagnostic use), Iodine Radioisotopes (diagnostic use), Kinetics, Lymphocytes (cytology), Lymphocytes (physiology), Macrophages, Peritoneal (cytology), Macrophages, Peritoneal (physiology), Mice, Mice, Inbred BALB C, Organic Chemicals, Organometallic Compounds (diagnostic use), Organometallic Compounds (pharmacokinetics), Oxyquinoline (analogs & derivatives), Oxyquinoline (diagnostic use), Oxyquinoline (pharmacokinetics), Radioisotope Dilution Technique, Time Factors, Tissue Distribution.
- MESH :
- chemical , analogs & derivatives : Oxyquinoline.
- chemical , diagnostic use : Fluorescent Dyes, Indium Radioisotopes, Iodine Radioisotopes, Organometallic Compounds, Oxyquinoline.
- cytology : Erythrocytes, Lymphocytes, Macrophages, Peritoneal.
- chemical , pharmacokinetics : Fluorescent Dyes, Organometallic Compounds, Oxyquinoline.
- physiology : Erythrocytes, Lymphocytes, Macrophages, Peritoneal.
- Animals, Cell Survival, Cells, Cultured, Kinetics, Mice, Mice, Inbred BALB C, Organic Chemicals, Radioisotope Dilution Technique, Time Factors, Tissue Distribution.
Abstract
Mouse macrophages purified by elutriation from thioglycollate-induced peritoneal exudate cells were labelled with indium-111-oxine and injected intravenously into mice. A substantial amount of unbound radioactivity remained in the circulation, suggesting that the radionuclide was not stably bound to the cells. Culture experiments with radiolabelled cells showed that indium-111 was released in the medium. Another cell marker, PKH-95, an iodine-125-labelled aliphatic compound insertable into the cell membrane, bound more stably than indium-111. Five minutes after injection of 125I-PKH-95-labelled macrophages, about 98% of the cells were in a non-circulating pool. It was checked that PKH-95 labelling did not compromise the viability and functions of the macrophages and that autologous erythrocytes and blood mononuclear cells labelled with PKH-95 remained in the circulation after i.v. injection. One hour after injection, 125I-PKH-95-labelled macrophages were distributed mainly in lung (36%), liver (19%) and spleen (5%). Subsequently, radioactivity decreased in the lung while increasing in liver, spleen and in an artificially induced footpad inflammation. The radioactivity accumulation in the inflammation persisted at least for 7 days. It represented a small proportion of radioactivity injected (0.2%) but was trapped very specifically in the inflammation. This raised the hypothesis that macrophages of the non-circulating pool could be released in the circulation and recruited into the inflammation with slow kinetics.
PubMed: 8535344
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fate of mouse macrophages radiolabelled with PKH-95 and injected intravenously.</title><author><name sortKey="Audran, R" uniqKey="Audran R">R Audran</name><affiliation wicri:level="1"><nlm:affiliation>Centre régional de lutte contre le cancer, Rennes, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre régional de lutte contre le cancer, Rennes</wicri:regionArea><placeName><region type="région">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Collet, B" uniqKey="Collet B">B Collet</name></author><author><name sortKey="Moisan, A" uniqKey="Moisan A">A Moisan</name></author><author><name sortKey="Toujas, L" uniqKey="Toujas L">L Toujas</name></author></titleStmt><publicationStmt><date when="1995">1995</date><idno type="RBID">pubmed:8535344</idno><idno type="pmid">8535344</idno><idno type="wicri:Area/Main/Corpus">004922</idno><idno type="wicri:Area/Main/Curation">004922</idno><idno type="wicri:Area/Main/Exploration">004716</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Survival</term><term>Cells, Cultured</term><term>Erythrocytes (cytology)</term><term>Erythrocytes (physiology)</term><term>Fluorescent Dyes (diagnostic use)</term><term>Fluorescent Dyes (pharmacokinetics)</term><term>Indium Radioisotopes (diagnostic use)</term><term>Iodine Radioisotopes (diagnostic use)</term><term>Kinetics</term><term>Lymphocytes (cytology)</term><term>Lymphocytes (physiology)</term><term>Macrophages, Peritoneal (cytology)</term><term>Macrophages, Peritoneal (physiology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Organic Chemicals</term><term>Organometallic Compounds (diagnostic use)</term><term>Organometallic Compounds (pharmacokinetics)</term><term>Oxyquinoline (analogs & derivatives)</term><term>Oxyquinoline (diagnostic use)</term><term>Oxyquinoline (pharmacokinetics)</term><term>Radioisotope Dilution Technique</term><term>Time Factors</term><term>Tissue Distribution</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Oxyquinoline</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Fluorescent Dyes</term><term>Indium Radioisotopes</term><term>Iodine Radioisotopes</term><term>Organometallic Compounds</term><term>Oxyquinoline</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Erythrocytes</term><term>Lymphocytes</term><term>Macrophages, Peritoneal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Fluorescent Dyes</term><term>Organometallic Compounds</term><term>Oxyquinoline</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Erythrocytes</term><term>Lymphocytes</term><term>Macrophages, Peritoneal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Survival</term><term>Cells, Cultured</term><term>Kinetics</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Organic Chemicals</term><term>Radioisotope Dilution Technique</term><term>Time Factors</term><term>Tissue Distribution</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mouse macrophages purified by elutriation from thioglycollate-induced peritoneal exudate cells were labelled with indium-111-oxine and injected intravenously into mice. A substantial amount of unbound radioactivity remained in the circulation, suggesting that the radionuclide was not stably bound to the cells. Culture experiments with radiolabelled cells showed that indium-111 was released in the medium. Another cell marker, PKH-95, an iodine-125-labelled aliphatic compound insertable into the cell membrane, bound more stably than indium-111. Five minutes after injection of 125I-PKH-95-labelled macrophages, about 98% of the cells were in a non-circulating pool. It was checked that PKH-95 labelling did not compromise the viability and functions of the macrophages and that autologous erythrocytes and blood mononuclear cells labelled with PKH-95 remained in the circulation after i.v. injection. One hour after injection, 125I-PKH-95-labelled macrophages were distributed mainly in lung (36%), liver (19%) and spleen (5%). Subsequently, radioactivity decreased in the lung while increasing in liver, spleen and in an artificially induced footpad inflammation. The radioactivity accumulation in the inflammation persisted at least for 7 days. It represented a small proportion of radioactivity injected (0.2%) but was trapped very specifically in the inflammation. This raised the hypothesis that macrophages of the non-circulating pool could be released in the circulation and recruited into the inflammation with slow kinetics.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">8535344</PMID><DateCreated><Year>1996</Year><Month>02</Month><Day>08</Day></DateCreated><DateCompleted><Year>1996</Year><Month>02</Month><Day>08</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0969-8051</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>6</Issue><PubDate><Year>1995</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Nuclear medicine and biology</Title><ISOAbbreviation>Nucl. Med. Biol.</ISOAbbreviation></Journal><ArticleTitle>Fate of mouse macrophages radiolabelled with PKH-95 and injected intravenously.</ArticleTitle><Pagination><MedlinePgn>817-21</MedlinePgn></Pagination><Abstract><AbstractText>Mouse macrophages purified by elutriation from thioglycollate-induced peritoneal exudate cells were labelled with indium-111-oxine and injected intravenously into mice. A substantial amount of unbound radioactivity remained in the circulation, suggesting that the radionuclide was not stably bound to the cells. Culture experiments with radiolabelled cells showed that indium-111 was released in the medium. Another cell marker, PKH-95, an iodine-125-labelled aliphatic compound insertable into the cell membrane, bound more stably than indium-111. Five minutes after injection of 125I-PKH-95-labelled macrophages, about 98% of the cells were in a non-circulating pool. It was checked that PKH-95 labelling did not compromise the viability and functions of the macrophages and that autologous erythrocytes and blood mononuclear cells labelled with PKH-95 remained in the circulation after i.v. injection. One hour after injection, 125I-PKH-95-labelled macrophages were distributed mainly in lung (36%), liver (19%) and spleen (5%). Subsequently, radioactivity decreased in the lung while increasing in liver, spleen and in an artificially induced footpad inflammation. The radioactivity accumulation in the inflammation persisted at least for 7 days. It represented a small proportion of radioactivity injected (0.2%) but was trapped very specifically in the inflammation. This raised the hypothesis that macrophages of the non-circulating pool could be released in the circulation and recruited into the inflammation with slow kinetics.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Audran</LastName><ForeName>R</ForeName><Initials>R</Initials><Affiliation>Centre régional de lutte contre le cancer, Rennes, France.</Affiliation></Author><Author ValidYN="Y"><LastName>Collet</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Moisan</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Toujas</LastName><ForeName>L</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>ENGLAND</Country><MedlineTA>Nucl Med Biol</MedlineTA><NlmUniqueID>9304420</NlmUniqueID><ISSNLinking>0969-8051</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Fluorescent Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Iodine Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Organic Chemicals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>PKH 95</NameOfSubstance></Chemical><Chemical><RegistryNumber>14514-42-2</RegistryNumber><NameOfSubstance>indium oxine</NameOfSubstance></Chemical><Chemical><RegistryNumber>5UTX5635HP</RegistryNumber><NameOfSubstance>Oxyquinoline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Erythrocytes</DescriptorName><QualifierName MajorTopicYN="N">cytology</QualifierName><QualifierName MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Fluorescent Dyes</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName><QualifierName MajorTopicYN="N">pharmacokinetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium Radioisotopes</DescriptorName><QualifierName MajorTopicYN="N">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Iodine Radioisotopes</DescriptorName><QualifierName MajorTopicYN="N">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Lymphocytes</DescriptorName><QualifierName MajorTopicYN="N">cytology</QualifierName><QualifierName MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Macrophages, Peritoneal</DescriptorName><QualifierName MajorTopicYN="N">cytology</QualifierName><QualifierName MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Organic Chemicals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Organometallic Compounds</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName><QualifierName MajorTopicYN="N">pharmacokinetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oxyquinoline</DescriptorName><QualifierName MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName MajorTopicYN="N">diagnostic use</QualifierName><QualifierName MajorTopicYN="N">pharmacokinetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Radioisotope Dilution Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tissue Distribution</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>8</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8535344</ArticleId><ArticleId IdType="pii">0969-8051(95)00013-N</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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